The osteogenesis of bacterial cellulose scaffold loaded with bone morphogenetic protein-2

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• 作者：Qin Shi ; Yang Li ; Jie Sun ; Hua Zhang ; Lei Chen ; Bing Chen ; Huilin Yang ; Zhaoxu Wang
• 关键词：Osteogenesis ; Bacterial cellulose scaffold ; Bone morphogenetic protein-2 ; Nanofibrous
• 刊名：Biomaterials
• 出版年：2012
• 出版时间：October, 2012
• 年：2012
• 卷：33
• 期：28
• 页码：6644-6649
• 全文大小：1256 K

文摘

Bacterial cellulose (BC) is a nanofibrous biological material with attractive physicochemical properties and biocompatibility. Its fiber is similar to the collagenous fiber of bone. To explore if BC could be utilized as a localized delivery system to increase the local concentration of cytokines for tissue engineering, we prepared the BC scaffold from Acetobacter xylinum X-2 (A.?xylinum X-2) and investigated the osteogenic potential of the BC scaffold coated with bone morphogenetic protein-2 (BMP-2). The data showed that BC had a good biocompatibility and induced differentiation of mouse fibroblast-like C2C12 cells into osteoblasts in the presence of BMP-2 in?vitro, as demonstrated by alkaline phosphatase (ALP) activity assays. Within a certain range (0?¡«?3?¦Ìg/scaffold), the osteogenic activity of induced osteoblasts was positively correlated to the concentrations of BMP-2. In in?vivo subcutaneous implantation studies, BC scaffolds carrying BMP-2 showed more bone formation and higher calcium concentration than the BC scaffolds alone at 2 and 4 weeks, respectively. The ALP activity assay and the measurement of calcium concentration of BC scaffolds also showed that more new bone was developed in the BC scaffolds carrying BMP-2 than in the BC scaffolds alone. Our studies suggest that BC is a good localized delivery system for BMPs and would be a potential candidate in bone tissue engineering.