Effects of the interaction of TiO₂ nanoparticles with bisphenol A on their physicochemical properties and in vitro toxicity

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• 作者：Dan Zheng^a ; ^{zhengdan@mails.tjmu.edu.cn} ; Nan Wang^b ; ^{wnanhust@yahoo.com.cn} ; Xinmei Wang^a ; ^{wangzizi33@Tom.Com} ; Ying Tang^c ; ^{maotou01@163.com} ; Lihua Zhu^b ; ^{lhzhu63@yahoo.com.cn} ; Zheng Huang^a ; ^{huangzhg@mails.tjmu.edu.cn} ; Heqing Tang^b ; ^{hqtang62@yahoo.com.cn} ; Yun Shi^a ; ^{yunshi31@163.com} ; Yating Wu^a ; ^{15095023@qq.com} ; Meng Zhang^a ; ^{zm20080102@126.com} ; Bin Lu^a ; ^{lubin@mails.tjmu.edu.cn}
• 关键词：Nano-TiO2 ; titanium dioxide nanoparticles ; BPA ; bisphenol A ; DMEM ; Dulbecco's minimum essential eagle's medium ; TEM ; transmission electron microscopy ; FT-IR ; Fourier transform infrared spectrophotometer ; DCFH-DA ; 2 ; 7-dichlorofluorescin diacetate ; MN ; micr
• 刊名：Journal of Hazardous Materials
• 出版年：2012
• 出版时间：15 January, 2012
• 年：2012
• 卷：199-200
• 期：Complete
• 页码：426-432
• 全文大小：755 K

文摘

In this paper we evaluated the effects of the interaction of TiO₂ nanoparticles (nano-TiO₂) with bisphenol A (BPA) on their physicochemical properties and in vitro toxicity in human embryo L-02 hepatocytes. Different concentrations of BPA (0, 0.1, 1, 10 ¦Ìmol/L) and nano-TiO₂ (0, 0.1, 1, 10 mg/L) were mixed to analyze the size distribution, zeta potential, adsorption capacity and uptake of nano-TiO₂, and the toxicity of nano-TiO₂ and BPA in L-02 cells. The addition of BPA to nano-TiO₂ dispersions increased the aggregation level and zeta potential of nano-TiO₂ in all media. Nano-TiO₂ had a similar adsorption capacity in different media, although a higher aggregation level was observed in cell culture medium. Nano-TiO₂, with or without BPA, could enter L-02 cells after 24 h exposure. Nano-TiO₂ alone did not induce significant DNA and chromosome damage, but the mixture of nano-TiO₂ and BPA increased toxicity via increasing oxidative stress, DNA double strand breaks and micronuclei formation. The aggregated nano-TiO₂ can enrich BPA effectively. The BPA-bound nano-TiO₂ are proven to be uptaken into nuclei of exposed cells, which may increase intracellular BPA and nano-TiO₂ levels and thus lead to synergistic toxicity. However only small synergic effects were observed at the concentrations of BPA and nano-TiO₂ used in this study.