Nanog interact with CDK6 to regulates astrocyte cells proliferation following spinal cord injury

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• 作者：Jun Gu^a ; ^b ; Yingjie Ni^b ; Lin Xu^b ; Hongliang Xu^b ; Zhengdong Cai^a ; ^{caizhengdongsh@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Nanog ; CDK6 ; Spinal cord ; Astrocyte ; Proliferation
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：22 January 2016
• 年：2016
• 卷：469
• 期：4
• 页码：1097-1103
• 全文大小：2606 K

文摘

Previous research had reported transcription factors Nanog expressed in pluripotent embryonic stem cells (ESCS) that played an important role in regulating the cell proliferation. Nanog levels are frequently elevated in ESCS, but the role in the spinal cord was not clear. To examine the biological relevance of Nanog, we studied its properties in spinal cord injury model. The expression of Nanog and PCNA was gradually increased and reached a peak at 3 day by western blot analysis. The expression of Nanog was further analyzed by immunohistochemistry. Double immunofluorescent staining uncovered that Nanog can co-labeled with PCNA and GFAP in the spinal cord tissue. In vitro, Nanog can promote the proliferation of astrocyte cell by Fluorescence Activating Cell Sorter (FACS) and CCK8. Meanwhile, the cell-cycle protein CDK6 could interact with Nanog in the spinal cord tissue. Taken together, these data suggested that both Nanog may play important roles in spinal cord pathophysiology via interact with CDK6.