Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)

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• 作者：Qiushi Wang ; Xianchun Sang ; Yinghua Ling ; Fangming Zhao ; Zhenglin Yang ; Yunfeng Li ; Guanghua He
• 关键词：molecular mapping ; rice (Oryza sativa L.) ; zebra mutant ; chlorophyll ; Zebra-15
• 刊名：Journal of Genetics and Genomics
• 出版年：2009
• 出版时间：November 2009
• 年：2009
• 卷：36
• 期：11
• 页码：679-684
• 全文大小：323 K

文摘

A novel zebra mutant, zebra-15, derived from the restorer line Jinhui10 (Oryza sativa L. ssp. indica) treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased (55.4 % ) and the ratio of Chla/Chlb increased (90.2 % ) significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F₂ segregation populations derived from the cross of Xinong1A and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the Xinong1A and the zebra-15, their F₂ population was then used for gene mapping. Zebra-15 (Z-15) gene was primarily restricted on the short arm of chromosome 5 by 150 F₂ recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F₂ recessive individuals.