NF-κB/Rel, not STAT5, regulates nitric oxide synthase transcription in Apostichopus japonicus
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- 作者：Yina Shao ; Zhenhui Wang ; Zhimeng Lv ; Chenghua Li ; lichenghua@nbu.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Weiwei Zhang ; Ye Li ; Xuemei Duan
- 关键词：Apostichopus japonicus ; NOS promoter ; Transcription factor ; Promoter activity analysis ; Luciferase assay
- 刊名：Developmental & Comparative Immunology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：61
- 期：Complete
- 页码：42-47
- 全文大小：857 K
文摘
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Promoter region of nitric oxide synthase were identified in Apostichopus japonicus by genome walking.
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Luciferase assay indicated the region −614/+39 contributed importantly to expression of the AjNOS.
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The first NF-κB binding site (−375 bp to −366 bp) was essential to induce AjNOS transcription.
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STAT5 could not activate AjNOS expression in EPC cell.
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NF-κB inhibitor SN50 suppressed the AjNOS mRNA expression in cultured coelomocytes.