Correlation between miR-23a and onset of hepatocellular carcinoma

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• 作者：Lidao Bao^a ; ¹ ; ^{lidao_bao@dfci.harvard.edu" class="auth_mail} ; Jianfen Zhao^b ; Xiaoxia Dai^c ; Yi Wang^a ; Ruilian Ma^a ; Yila Su^d ; Hongwei Cui^d ; Jianxiang Niu^e ; Shiming Bai^f ; Zhiying Xiao^g ; Hongwei Yuan^h ; Zhou Yangⁱ ; Changqing Li^j ; Rui Cheng^a ; Xianhua Ren^a ; ^{renxianhua237@163.com" class="auth_mail}
• 刊名：Clinics and Research in Hepatology and Gastroenterology
• 出版年：June 2014
• 年：2014
• 卷：38
• 期：3
• 页码：318-330
• 全文大小：2412 K

文摘

To clarify the role of miR-23a in the onset and development of hepatocarcinoma on the cellular, genetic and molecular levels.

Patients and methods

Seventy-eight patients were included after hepatectomy. Relationships between the clinical pathological factors of tumor and paracancerous tissues were analyzed. Risk factors of overall and recurrence-free survival rates were subject to multi-variable analysis. Tissues were sequenced by digital miRNA expression profiling, and new miRNA was subject to target gene prediction.

Results

miR-23a expression was correlated with the stage of the TNM Classification of Malignant Tumours most significantly, followed by tumor size (P = 0.041 and 0.047). High miR-23a, vascular invasion, tumor size ≥ 7 cm, tumor capsule and late pathological stage were the risk factors of overall survival rate, and those of recurrence-free survival rate also included alpha-fetoprotein level ≥ 200 渭g/L and multiple tumors. Compared with normal hepatic cell line L-02, the miR-23a expression levels in tumor cell lines SMMC-7721 and HepG2 were up-regulated and down-regulated respectively. Transfecting miR-23a inhibitor suppressed cell growth. Apoptotic rates of the control and those transfected with inhibitor-NC and miR-23a inhibitor for 48 h were similar.

Conclusion

High miR-23a expression is the independent prognostic factor of overall and recurrence-free survival rates, and miR-23a may be involved in the onset of hepatocarcinoma as an oncogene.