Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout

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• 作者：Xiaoming Ma ; Zhonghui Chen ; Jin Zhou ; Wen Weng ; Ou Zheng ; Zhenyu Lin ; Longhua Guo ; Bin Qiu ; Guonan Chen
• 关键词：Personal glucose meters ; Aptamer ; Biosensor ; Platelet-derived growth factor B
• 刊名：Biosensors and Bioelectronics
• 出版年：15 May, 2014
• 年：2014
• 卷：55
• 期：Complete
• 页码：412-416
• 全文大小：1035 K

文摘

A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0脳10^-14 M~3.16脳10^鈭?2 M, and the detection limit is 2.9 fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples.