Extraction, purification and characterization of polysaccharides from Hawk tea
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- 作者：Xuejing Jia ; Chunbang Ding ; Shu Yuan ; Zhongwei Zhang ; Yang鈥檈r Chen ; Lei Du ; Ming Yuan
- 关键词：Litsea coreana var. lanuginose ; Polysaccharide ; Response surface methodology ; Antioxidant activity
- 刊名：Carbohydrate Polymers
- 出版年：2 January, 2014
- 年：2014
- 卷：99
- 期：Complete
- 页码：319-324
- 全文大小：1728 K
文摘
In the present study, the extraction, purification and characterization of polysaccharides from Hawk mature leaf tea (HMP) were investigated. The optimal extraction parameters were obtained by using a Box-Behnken design as follows: extraction temperature 88.9 掳C, extraction time 128.2 min and ratio of water to solid 11.4 mL/g. The crude HMP was sequentially purified by chromatography of DEAE-52, and two purified fractions, HMP-1 and HMP-2, were obtained. HMP-1 and HMP-2 were mainly composed of arabinose, galactose, glucose and mannose with the molecular weight of 133 and 100 kDa, respectively. For antioxidant activities in vitro, HMP-1 had strong 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging activity and ferric reducing activity power (FRAP). These results provide a scientific basis for the further use of polysaccharides from this traditional herb tea.