Knockout of microRNA-155 ameliorates the Th1/Th17 immune response and tissue injury in chronic rejection

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• 作者：Anchen Zhang ; PhD^a ; ^d ; ¹ ; Ke Wang ; PhD^a ; ¹ ; Cheng Zhou ; PhD^a ; ¹ ; Zheng Gan ; PhD^b ; ¹ ; Dongxia Ma ; PhD^c ; Ping Ye ; PhD^d ; Yuan Sun ; PhD^a ; Jie Wu ; PhD^a ; Xiaofan Huang ; PhD^a ; Lingyun Ren ; PhD^a ; Peng Deng ; PhD^a ; Chuangyan Wu ; PhD^a ; Zhang Yue ; PhD^a ; Xiangchao Ding ; PhD^a ; Jiuling Chen ; PhD^a ; Jiahong Xia ; MD ; PhD^a ; ^d ; ^e ; ^{jiahong.xia@mail.hust.edu.cn}
• 关键词：CAV ; miR-155 ; Th17 ; rejection ; heart transplantation
• 刊名：The Journal of Heart and Lung Transplantation
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：36
• 期：2
• 页码：175-184
• 全文大小：3467 K
• 卷排序：36

文摘

MicroRNAs (miRNAs) are integral for maintaining immune homeostasis and self-tolerance. The influence of miRNAs on T-cell differentiation and plasticity are critical in the development of chronic rejection of transplanted hearts. In this study, we sought to determine whether the knockout of miR-155 affects the development of cardiac allograft vasculopathy (CAV) in a murine model.MethodsmiRNA microarray and quantitative polymerase chain reaction (qPCR) analyses were performed for allograft neointimal lesion samples in chronic rejection. A model of heterotopic murine heart transplantation (bm12 to miR-155+/+ or miR-155–/– mice) was then used to analyze allograft survival, histology, mRNA expression and T-cell sub-populations in spleens. The accelerated experiments were performed by intraperitoneal injection of either recombinant interleukin-17A or phosphate-buffered saline (PBS) after heart transplantation. For the competitive transfer experiments, CD4+ splenocytes from wild-type (WT) or miR-155–/– mice were mixed and injected into Rag1–/– mice, and cardiac transplantation was performed after 24 hours. The differentiation of T-helper subsets (Th1/Th17/iTreg) was investigated in vitro.ResultsmiR-155–/– mice showed resistance to cardiac rejection along with weakened T-cell–mediated inflammation, especially for Th17 cells. Recombinant IL-17A could restore this relieved injury. The competitive experiments implied that miR-155 plays a vital role in the stability of the Th17 phenotype. In vitro, we also demonstrated that miR-155–/– mice exhibit a defect in Th17 differentiation.ConclusionsmiR-155 regulates Th1/Th17-related inflammation in chronic cardiac rejection and may be a potential therapeutic target to attenuate cardiac allograft rejection.Despite advancements in immunosuppressive therapy, the immunologic mechanisms responsible for allograft rejection remain an important issue for both clinicians and researchers. Allograft rejection is a T-cell–dependent phenomenon and is critically dependent on inflammation mediated by CD4+ Th subsets, including Th1, Th2, Th17, Th9 and regulatory T (Treg) cells.