Winter cover crops alter methanotrophs community structure in a double-rice paddy soil

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• 作者：Jing-na LIU^a ; ^* ; ^{liujingna199066@126.com" class="auth_mail" title="E-mail the corresponding author} ; Bo ZHU^b ; ^* ; ^{1984zhubo@163.com" class="auth_mail" title="E-mail the corresponding author} ; Li-xia YI^b ; Hong-cui DAI^a ; He-shui XU^a ; Kai ZHANG^a ; Yue-gao HU^a ; Zhao-hai ZENG^a ; ^{zengzhaohai@cau.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Chinese milk vetch ; ryegrass ; methanotrophic bacteria ; community structure ; double-rice
• 刊名：Journal of Integrative Agriculture
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：15
• 期：3
• 页码：553-565
• 全文大小：1226 K

文摘

Methanotrophs play a vital role in the mitigation of methane emission from soils. However, the influences of cover crops incorporation on paddy soil methanotrophic community structure have not been fully understood. In this study, the impacts of two winter cover crops (Chinese milk vetch (Astragalus sinicus L.) and ryegrass (Lolium multiflorum Lam.), representing leguminous and non-leguminous cover crops, respectively) on community structure and abundance of methanotrophs were evaluated by using PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and real-time PCR technology in a double-rice cropping system from South China. Four treatments were established in a completely randomized block design: 1) double-rice cropping without nitrogen fertilizer application, CK; 2) double-rice cropping with chemical nitrogen fertilizer application (200 kg ha^–1 urea for entire double-rice season), CF; 3) Chinese milk vetch cropping followed by double-rice cultivation with Chinese milk vetch incorporation, MV; 4) ryegrass cropping followed by double-rice cultivation with ryegrass incorporation, RG. Results showed that cultivating Chinese milk vetch and ryegrass in fallow season decreased soil bulk density and increased rice yield in different extents by comparison with CK. Additionally, methanotrophic bacterial abundance and community structure changed significantly with rice growth. Methanotrophic bacterial pmoA gene copies in four treatments were higher during late-rice season (3.18×10⁷ to 10.28×10⁷ copies g^–1 dry soil) by comparison with early-rice season (2.1×10⁷ to 9.62×10⁷ copies g^–1 dry soil). Type I methanotrophs absolutely predominated during early-rice season. However, the advantage of type I methanotrophs kept narrowing during entire double-rice season and both types I and II methanotrophs dominated at later stage of late-rice.