Inverse regulation of the interferon-γ receptor and its signaling in human endometrial stromal cells during decidualization

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• 作者：Herbert Fluhr ; Kristina Ramp ; Stefanie Krenzer ; Peter Licht ; Marek Zygmunt
• 关键词：Endometrium ; decidualization ; implantation ; IFN-γ ; R ; hCG
• 刊名：Fertility and Sterility
• 出版年：2009
• 出版时间：May 2009
• 年：2009
• 卷：91
• 期：5, Supplement 1
• 页码：2131-2136
• 全文大小：270 K

文摘

Objective

To investigate whether human endometrial stromal cells (ESCs) express the interferon-γ-receptor (IFN-γR) and whether the process of decidualization or human chorionic gonadotropin (hCG) regulate the IFN-γR and its signaling pathway.

Design

In vitro experiment.

Setting

Research laboratory at a medical university center.

Patient(s)

Premenopausal women undergoing hysterectomy for benign reasons.

Intervention(s)

Isolation and incubation of ESCs from hysterectomy specimens with 17β-estradiol, progesterone, recombinant hCG, and IFN-γ as well as an IFN-γR-blocking antibody.

Main Outcome Measure(s)

We analyzed IFN-γR and the phosphorylation of signal transducer and activator of transcription 1 (STAT-1) by flow cytometry. We measured IFN-γR and interferon response factor 1 (IRF-1) mRNA using semiquantitative real-time reverse transcriptase polymerase chain reaction (RT-PCR).

Result(s)

The IFN-γR is up-regulated in human ESCs during decidualization without affecting the phosphorylation of STAT-1. Stimulation of IRF-1 by IFN-γ is reduced in decidualized ESCs. We found that hCG neither regulates the IFN-γR nor its signaling pathway.

Conclusion(s)

These results show an inverse regulation of the IFN-γR and its signaling response via STAT-1 and IRF-1 in human ESCs during decidualization. The early embryonic signal hCG has no effect on this process. This mechanism may finely modulate the reactivity of ESCs to IFN-γ-mediated signals from immune cells at the implantation site.