Exosome cargo reflects TGF-β1-mediated epithelial-to-mesenchymal transition (EMT) status in A549 human lung adenocarcinoma cells

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• 作者：Jiyeon Kim^a ; Tae Yeon Kim^b ; Myung Shin Lee^c ; Ji Young Mun^d ; Chunhwa Ihm^e ; Soon Ae Kim^b ; ^{sakim@eulji.ac.kr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Exosome ; Epithelial to mesenchymal transition ; Transforming growth factor β1 ; Lung adenocarcinoma ; MicroRNA
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2016
• 出版时间：16 September 2016
• 年：2016
• 卷：478
• 期：2
• 页码：643-648
• 全文大小：905 K

文摘

It has been suggested that tumor cells secrete exosomes to modify the local microenvironment, which then promotes intercellular communication and metastasis. Although exosomes derived from cancer cells may contribute to the epithelial–mesenchymal transition (EMT) in untransformed cells, few studies have defined exosome cargo upon induction of EMT. In this study, we investigated the changes in exosomal cargo from the epithelial to mesenchymal cell phenotype by inducing EMT with transforming growth factor (TGF)-β1 in A549 human lung adenocarcinoma cells. The protein content of the exosomes reflects the change in the cell phenotype. In addition, miR-23a was significantly enriched in the exosomes after mesenchymal transition. Following treatment of exosomes from mesenchymal cells via EMT induction with TGF-β1 to the epithelial cell type, phenotypic changes in protein expression level and cell morphology were observed. Autologous treatment of exosomes enhanced the transcriptional activity and abundance of β-catenin. Our results suggest that the exosomal protein and miRNA content reflects the physiological condition of its source and that exosomes induce phenotypic changes via autocrine signaling.