Cloning and characterization of d-threonine aldolase from the green alga Chlamydomonas reinhardtii

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• 作者：Yuki Hirato^a ; Mayumi Tokuhisa^a ; Minoru Tanigawa^a ; Hiroyuki Ashida^b ; Hiroyuki Tanaka^c ; Katsushi Nishimura^a ; ^d ; ^{nishimura.katsushi@nihon-u.ac.jp}
• 关键词：Chlamydomonas reinhardtii ; Chlamydomonadaceae ; Green alga ; Enzyme characterization ; d-Amino acid ; d-Threonine aldolase ; Pyridoxal 5&rsquo ; -phosphate
• 刊名：Phytochemistry
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：135
• 期：Complete
• 页码：18-23
• 全文大小：1479 K
• 卷排序：135

文摘

This is the first d-threonine aldolase characterized from a Eukaryote. d-Threonine aldolase of Chlamydomonas reinhardtii (CrDTA) was cloned and expressed in Escherichia coli. CrDTA catalyzed cleavage and synthetic reactions of β-hydroxy-d-amino acids.