Neuroprotection and antioxidative effects of Sijunzi Tang Decoction in the nematode Caenorhabditis elegans

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• 作者：Andrea Schlotterer^a ; ¹ ; ^{Andrea.Schlotterer@medma.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author} ; Henry Johannes Greten^b ; ^c ; ¹ ; ^{HeidelbergSchool@aol.com" class="auth_mail" title="E-mail the corresponding author} ; Bjoern Andrew Remppis^d ; ^{ba.remppis@hgz-bb.de" class="auth_mail" title="E-mail the corresponding author} ; Georgi Kukudov^a ; ^{Georgi.Kukudov@medma.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author} ; Thomas Efferth^e ; ^{efferth@uni-mainz.de" class="auth_mail" title="E-mail the corresponding author} ; Jorge Machado^b ; ^f ; ^{jmachado@icbas.up.pt" class="auth_mail" title="E-mail the corresponding author} ; Per Humpert^g ; ^h ; ^{humpert@swzrp.de" class="auth_mail" title="E-mail the corresponding author} ; Hans-Peter Hammes^a ; ^{Hans-Peter.Hammes@medma.uni-heidelberg.de" class="auth_mail" title="E-mail the corresponding author} ; Michael Morcos^a ; ^h ; ^{morcos@swzrp.de" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ANOVA ; analysis of variance ; AU ; arbitrary unit ; HG ; high glucose ; LWDHD ; Liuwei Dihuang Decoction ; PGG ; 1 ; 2 ; 3 ; 4 ; 6-pentagalloyl glucose ; PLSD ; Fisher&rsquo ; s protected least significant difference ; ROS ; reactive oxygen species ; S ; standard ; SJZTD ; Sijunzi Tang Decoction ; SWTD ; Si-Wu-Tang Decoction ; TCM ; traditional Chinese medicine ; UT ; untreated
• 刊名：European Journal of Integrative Medicine
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：8
• 期：4
• 页码：526-532
• 全文大小：703 K

文摘

Knowledge about the pharmacological properties of traditional TCM formulas is scarce as is their impact on aging. This study evaluated the potential effects of Sijunzi Tang Decoction (SJZTD), Si-Wu-Tang Decoction (SWTD), and Liuwei Dihuang Decoction (LWDHD) against oxidative stress and glucotoxic stress-induced neuronal dysfunction and lifespan reduction.

Methods

Nematodes (C. elegans) were cultivated under 10 mmol/L paraquat or 400 mmol/L glucose to assess the protective effects of a systemic Traditional Chinese Medicine (TCM) treatment. Lifespan was determined by calculation of Kaplan-Meier survival curves, formation of reactive oxygen species was quantified by hydroethidine staining, and neuronal functions were evaluated by worm tracking analysis.

Results

A 10% SJZTD solution largely normalized nematode survival under both oxidative stress (increased 3-day-survival rate by 143%) and glucotoxic stress (extended mean lifespan from 19.90 to 23.71 days). Under high glucose conditions we demonstrated both antioxidative effects (reduced Reactive Oxygen Species (ROS) formation by 71%) and neuroprotective effects (improved whole nematode motility by 179%). SWTD and LWDHD failed to confer protection in our model system.

Conclusions

The neuroprotective effects for SJZT decoction against glucotoxic stress due to pronounced antioxidant properties were demonstrated. Future studies should be extended to protection of the neurovascular system in higher organisms.