Prognostic Significance of MAGE-A11 in Esophageal Squamous Cell Carcinoma and Identification of Related Genes Based on DNA Microarray

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• 作者：Meixiang Sang^a ; ^b ; Lina Gu^a ; Fei Liu^a ; Yishui Lian^a ; Danjing Yin^a ; Xiaojie Fan^a ; Chunyan Ding^a ; Weina Huang^a ; Shina Liu^a ; Baoen Shan^a ; ^b ; ^{baoenshan@hotmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Esophageal squamous cell carcinoma (ESCC) ; Melanoma-associated antigens (MAGE) ; MAGE-A11 ; Prognosis ; Microarray
• 刊名：Archives of Medical Research
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：47
• 期：3
• 页码：151-161
• 全文大小：2066 K

文摘

This study aims to investigate the expression pattern of melanoma-associated antigen-A11 (MAGE-A11) in esophageal squamous cell carcinoma (ESCC) specimens and analyze its prognostic significance for ESCC patients. In addition, the purpose of our study was also to explore the biological function of MAGE-A11 in ESCC cells based on DNA microarray.

Methods

Immunohistochemistry was used to detect the expression of MAGE-A11 in ESCC specimens, and its prognostic significance was analyzed by statistical analysis. DNA microarray and quantitative RT-PCR were used to explore the different expression of MAGE-A11 downstream genes in ESCC cells. Cell invasion assay and MTT assay were used to detect the effect of MAGE-A11 cDNA on the invasion and proliferation of ESCC cells.

Results

Of the ESCC specimens, 59.3% showed positive MAGE-A11 expression. MAGE-A11 expression in ESCC specimens was positively associated with distant lymph node metastasis. Overall survival of ESCC patients with positive MAGE-A11 expression was shorter than in patients with negative MAGE-A11 expression. Multivariate Cox regression analysis showed MAGE-A11 expression is an independent poor prognostic factor for ESCC patients. Overexpression of MAGE-A11 changed a variety of gene expressions, which was associated with various cell functions such as protein ubiquitination, cell proliferation and apoptosis, tumor invasion and metastasis. Overexpression of MAGE-A11 directly increased the invasion and proliferation of ESCC cells.

Conclusions

MAGE-A11 is an independent poor prognostic marker for ESCC patients. MAGE-A11 regulates various cell functions and directly increases the invasion and proliferation of ESCC cells.