An improved amperometric L-lactate biosensor based on covalent immobilization of microbial lactate oxidase onto carboxylated multiwalled carbon nanotubes/copper nanoparticles/polyaniline modified pencil graphite electrode
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- 作者：Kusum Dagar ; C.S. Pundir ; pundrics@rediffmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：l-Lactate oxidase ; Lactic acid ; Copper nanoparticles ; Carboxylated multiwalled carbon nanotubes ; Polyaniline ; Pencil graphite electrode
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：177-186
- 全文大小：2072 K
文摘
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Constructed amperometric lactate biosensor based on microbial LOx/cMWCNT/CuNPs/PANI/PGE.
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Biosensor showed improved analytic performance e.g. 5 s response time, LOD- 0.25 μM and broader linear range 1 μM–2500 μM.
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Biosensor measured lactic acid in plasma, milk products, alcoholic beverages and fruit juice.
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Biosensor used 180 times over a period of 140 days, with only 40% loss in its initial activity.
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Biosensor exhibited good correlation with standard method.