Dietary Supplementation with a Combination of Lactoferrin, Fish Oil, and Enterococcus faecium WB2000 for Treating Dry Eye: A Rat Model and Human Clinical Study

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• 作者：Motoko Kawashima ; MD ; PhD¹ ; ^{motoko-k@a3.keio.jp" class="auth_mail" title="E-mail the corresponding author} ; Shigeru Nakamura ; DVM ; PhD¹ ; Yusuke Izuta ; MS¹ ; Sachiko Inoue ; MD¹ ; ² ; Kazuo Tsubota ; MD ; PhD¹
• 关键词：dietary supplements ; dry eye ; Schirmer test ; tears
• 刊名：The Ocular Surface
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：14
• 期：2
• 页码：255-263
• 全文大小：828 K

文摘

To examine the effect of a combined dietary supplement containing fish oil, lactoferrin, zinc, vitamin C, lutein, vitamin E, γ-aminobutanoic acid, and Enterococcus faecium WB2000 on dry eye.

Methods

A preliminary study in a rat model and a prospective, randomized, double-blind, placebo-controlled study in humans were conducted. Forty Japanese volunteers aged 22 to 59 years were randomized into combined dietary supplement (2 capsules/day; 20 participants) and placebo (vehicle; 19 participants) groups and treated once daily for 8 weeks. Rats received the combined dietary supplement components (10 or 50 mg/kg orally) or vehicle (2% DMSO), and dry eye was mechanically induced for 2 days. Tear production was measured in rats after dry eye was induced. Humans were assessed at baseline and weeks 4 and 8 post-supplementation based on keratoconjunctival epithelial damage; fluorescein tear film breakup time; tear production; biochemical data; information regarding subjective dry eye symptoms by answering a questionnaire; and information regarding adverse events via medical interviews.

Results

Supplementation dose-dependently mitigated the decrease in tear production in rats. Among subjects with confirmed dry eye, clinical symptoms improved at weeks 4 and 8 more significantly in the supplementation group than in the placebo group (P<.05). The rate of increase in the Schirmer value was greater in the supplementation group. No adverse events occurred.

Conclusion

Supplementation improved objective and subjective dry eye symptoms.