Combination of nisin and ε-PL improved preservative quality of fresh-cut carrots. Nisin and ε-PL inhibited white blush and lignification of fresh-cut carrots. Nisin and ε-PL with chitosan coating inhibited C4H and 4CL activities in carrots. Nisin and ε-PL synergistcally suppressed the expression of DcC4H and Dc4CL genes.