The phytoecdysteroid β-ecdysone is genotoxic in Rodent Bone Marrow Micronuclei and Allium cepa L. Assays

详细信息    查看全文

• 作者：Camila Siqueira Neves^a ; ^{camila-neves@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Shaiany Sabrina Lopes Gomes^a ; ^{bioshaygomes@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Tatianne Rosa dos Santos^b ; ^{tatiannersantos@yahoo.com.br" class="auth_mail" title="E-mail the corresponding author} ; Mariana Macedo de Almeida^b ; ^{marimacedoa@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Yamara Oliveira de Souza^b ; ^{yamaraoliveirasouza@bol.com.br" class="auth_mail" title="E-mail the corresponding author} ; Raú ; l Marcel Gonzá ; lez Garcia^b ; ^{raul.garcia@ufjf.edu.br" class="auth_mail" title="E-mail the corresponding author} ; Wagner Campos Otoni^c ; ^{wcotoni@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Luciana Moreira Chedier^d ; ^{lmchedier@hotmail.com" class="auth_mail" title="E-mail the corresponding author} ; Lyderson Facio Viccini^a ; ^{lyderson.viccini@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; José ; Marcello Salabert de Campos^a ; ^{joseufjf@gmail.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：20-Hydroxyecdysone (PubChem CID ; 5459840) ; Methyl Methanesulfonate (PubChem CID ; 4156)
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：11 January 2016
• 年：2016
• 卷：177
• 期：Complete
• 页码：81-84
• 全文大小：465 K

文摘

In South America, the β-ecdysone ecdysteroid has been found in species of the genus Pfaffia Mart. Due to the similar morphology of its roots to the Panax ginseng C. A. Mey. (Korean ginseng), some species of this genus has been known as Brazilian ginseng and have been used as tonic and aphrodisiac, as well as for the treatment of diabetes and rheumatism.

Aim of the study

Here we report a cytogenotoxic evaluation of β-ecdysone (a natural ecdysteroid found in plants) in Rodent Bone Marrow Micronuclei and Allium cepa Assays.

Materials and methods

Three β-ecdysone (pure) concentrations (based in human therapeutic dosage) were used in the Micronucleus Assay. The animals were treated during two consecutive days. Micronucleated cells were counted in 2000 polychromatic erythrocytes per animal. For A. cepa L. Assay, one β-ecdysone concentration was analyzed. The onions bulbs were exposed for 24 h.

Results

The Micronucleus Assay showed genotoxic effects for all treatments, expressed by an increase of micronucleated cells. In A. cepa L. Assay, cell abnormalities associated to the malfunction/non-formation of mitotic spindle (aneugenic effect) and chromosomal bridges (clastogenic effect) were observed.

Conclusions

The results indicate a cytogenotoxic activity of β-ecdysone. Therefore, the popular use of Pfaffia and others species containing β-ecdysone should be considered with caution.