DNA-duplex linker for AFM-SELEX of DNA aptamer against human serum albumin

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• 作者：Musashi Takenaka ; Yuzo Okumura ; Tomokazu Amino ; Yusuke Miyachi ; Chiaki Ogino ; ^{ochiaki@port.kobe-u.ac.jp} ; Akihiko Kondo
• 关键词：SELEX ; Atomic force microscope ; DNA aptamer ; Human serum albumin
• 刊名：Bioorganic & Medicinal Chemistry Letters
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：27
• 期：4
• 页码：954-957
• 全文大小：1108 K
• 卷排序：27

文摘

DNA-duplex interactions in thymines and adenins are used as a linker for the novel methodology of Atomic Force Microscope-Systematic Evolution of Ligands by EXpotential enrichment (AFM-SELEX). This study used the hydrogen bonds in 10 mer of both thymines (T10) and adenines (A10). Initially, the interactive force in T10-A10 was measured by AFM, which returned an average interactive force of approximately 350 pN. Based on this result, DNA aptamers against human serum albumin could be selected in the 4th round, and 15 different clones could be sequenced. The lowest dissociation constant of the selected aptamer was identified via surface plasmon resonance, and it proved to be identical to that of the commercial aptamer. Therefore, specific hydrogen bonds in DNA can be useful linkers for AFM-SELEX.