Ultrasensitive detection of mucin 1 biomarker by immuno-loop-mediated isothermal amplification

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• 作者：Hongmei Cao ; Xueen Fang ; ^{fxech@fudan.edu.cn} ; Haipeng Li ; Hua Li ; Jilie Kong ; ^{jlkong@fudan.edu.cn}
• 关键词：Mucin 1 ; Biomarker ; Immuno-loop-mediated isothermal amplification ; Low-abundance proteins
• 刊名：Talanta
• 出版年：2017
• 出版时间：1 March 2017
• 年：2017
• 卷：164
• 期：Complete
• 页码：588-592
• 全文大小：538 K
• 卷排序：164

文摘

The mucin 1 protein, as a tumor marker, is present in a variety of malignant tumors. Therefore, the sensitive and specific detection of mucin 1 has great significance for early cancer diagnoses. Report a novel technique of Immuno-Loop-Mediated Isothermal Amplification for the rapid quantitative detection of MUC-1 with high sensitivity (120 MUC-1 molecules by calculation) and specificity. Finally, this approach is also successfully applied in the analysis of human blood serum samples. It also lays the foundation for the early diagnosis of different types of low-abundance cancer biomarkers.