Advances in the 16α-hydroxy transformation of hydrocortisone by Streptomyces roseochromogenes
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- 作者：Odile Francesca Restaino ; odile.francesca@libero.it" class="auth_mail" title="E-mail the corresponding author ; odilefrancesca.restaino@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Mariacarmela Marseglia marimars84@hotmail.it" class="auth_mail" title="E-mail the corresponding author ; Paola Diana paola.diana@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Maria Giovanna Borzacchiello mariagiovanna.borzacchiello@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Rosario Finamore rosario.finamore@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Mario Vitiello mariovitiello.ita@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Antonella D&rsquo ; Agostino antonella.dagostino@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Mario De Rosa mario.derosa@unina2.it" class="auth_mail" title="E-mail the corresponding author ; Chiara Schiraldi ; chiara.schiraldi@unina2.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：16α-hydroxy hydrocortisone ; DO-stat fed-batch ; Reverse phase chromatography ; Steroid bio-transformation ; Streptomyces roseochromogenes
- 刊名：Process Biochemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：51
- 期：1
- 页码：1-8
- 全文大小：1251 K
文摘
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First-time studied pH and T effects on S. roseochromogenes 16α-hydroxylation of hydrocortisone.
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Conversion at 26 °C and pH 6 led to a 95% 16α-hydroxyhydrocortisone on total product ratio.
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Two-step substrate addition resulted suitable for 1.0 g/L hydrocortisone transformation.
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Fed-batch fermentation improved transformation up to 0.807 g/L of 16α-hydroxyhydrocortisone.
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Proposed reverse phase purification drove to 85% recovery of 16α-hydroxyhydrocortisone.
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