Development and evaluation of indirect ELISAs for the detection of IgG, IgM and IgA1 against duck hepatitis A virus 1
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- 作者：Sai Maoa ; b ; d ; 1 ; XuMin Oua ; b ; d ; 1 ; DeKang Zhua ; b ; c ; d ; 1 ; Shun Chena ; b ; c ; d ; 1 ; GuangPeng Mac ; d ; 1 ; MingShu Wanga ; b ; c ; d ; mshwang@163.com" class="auth_mail" title="E-mail the corresponding author ; RenYong Jiaa ; b ; c ; d ; MaFeng Liua ; b ; c ; d ; KunFeng Suna ; b ; c ; d ; Qiao Yanga ; b ; c ; d ; Ying Wua ; b ; c ; d ; XiaoYue Chenb ; d ; AnChun Chenga ; b ; c ; d ; chenganchun@vip.163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Duck hepatitis A virus 1 (DHAV-1) ; Indirect ELISAs (I-ELISAs) ; Linear regression equations ; IgG ; IgM ; IgA1
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：237
- 期：Complete
- 页码：79-85
- 全文大小：858 K
文摘
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Indirect ELISAs for detecting serum IgG, IgM and IgA1 against DHAV-1 were developed.
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Linear regression equations based on the I-ELISAs were derived.
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Regression equations were as effective as endpoint titrations in detecting antibodies.
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Analytical sensitivities of the indirect ELISAs were 1:2560, 1:1280 and 1:640 for IgG, IgM and IgA1, respectively.
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The concordances between I-ELISAs and serum-neutralization were 95.2% for IgG and IgA1, and 75% for IgM.