In vitro

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• 作者：Lihui Weng ; Natalia D. Ivanova ; Julia Zakhaleva ; Weiliam Chen
• 关键词：Hyaluronan ; Gelatin ; Microspheres ; Guanidinoethyl disulfide ; Cell infiltration
• 刊名：Biomaterials
• 出版年：2008
• 出版时间：November 2008
• 年：2008
• 卷：29
• 期：31
• 页码：4149-4156
• 全文大小：1305 K

文摘

This paper describes the preparation of oxidized hyaluronan crosslinked gelatin microspheres for drug delivery. Microspheres were prepared by a modified water-in-oil-emulsion crosslinking method, where three-dimensional crosslinked hydrogel microspheres formed in the absence of any extraneous crosslinker. SEM analyses of the microspheres showed rough surfaces in their dried state with an average diameter of 90 μm. Lyophilization of fully swollen microspheres revealed a highly porous structure. Guanidinoethyl disulfide (GED) was used as a model drug for incorporation into the microspheres; encapsulation of GED was confirmed by HPLC. There was an inverse correlation between the diameters of the microspheres with their GED loading. Macrophage was used as a model cell to evaluate the in vitro efficacy of GED release from the microspheres. The in vivo efficacy of the microspheres was further validated in a mouse full-thickness transcutaneous dermal wound model through suppression of cell infiltration.