Using internal ribosome entry sites to facilitate engineering of insect cells and used in secretion proteins production

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• 作者：Yi-Ting Lin^a ; Chao-Yi Teng^b ; Oliver B. Villaflores^c ; Ying-Ju Chen^b ; Ming-Kun Liu^d ; Hong-Lin Chan^d ; Tzyy-Rong Jinn^e ; Tzong-Yuan Wu^a ; ^f ; ^{tywu@cycu.edu.tw}
• 关键词：Baculovirus ; Internal ribosomal entry site ; Interferon-γ
• 刊名：Journal of the Taiwan Institute of Chemical Engineers
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：71
• 期：Complete
• 页码：13-19
• 全文大小：1702 K
• 卷排序：71

文摘

A non-lytic plasmid based bi-cistronic baculovirus-free insect cell expression was developed. PnV539 IRES but not RhPV IRES functioned well in Sf21 cells through the plasmid-based baculovirus-free insect cell expression system. Combined the PnV539 IRES with enhanced green fluorescent protein (EGFP) along with blasticidin-resistant gene to facilitate the isolation of the stably transformed insect cells.