Analysis of the Interaction of BCL9 with β-Catenin and Development of Fluorescence Polarization and Surface Plasmon Resonance Binding Assays for this Interaction

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• 作者：Steven A. Kawamoto ; Andrea D. Thompson ; Adriana Coleska ; Zaneta Nikolovska-Coleska ; Han Yi ; Shaomeng Wang
• 刊名：Biochemistry
• 出版年：2009
• 出版时间：October 13, 2009
• 年：2009
• 卷：48
• 期：40
• 页码：9534-9541
• 全文大小：871K
• 年卷期：v.48,no.40(October 13, 2009)
• ISSN：1520-4995

文摘

The transcriptional activator β-catenin is the primary mediator of the canonical Wnt signaling pathway and is frequently upregulated in many types of human cancer. Recent studies have suggested that the interaction of β-catenin and its cofactor, B-cell lymphoma 9 (BCL9), is crucial for its transcriptional activity. Targeting this interaction using small molecules will improve our understanding of the β-catenin/Wnt signaling pathway and may lead to the development of a new class of anticancer drugs. In this study, we developed a fluorescence polarization (FP)-based BCL9 binding assay. Using our initial FP assay, we performed extensive mutational analysis on four critical hydrophobic residues in the BCL9 peptide and determined the precise region in BCL9 responsible for binding to β-catenin. These results led to further optimization of our FP assay, making it amenable for high-throughput screening (HTS). We also developed and validated a complementary surface plasmon resonance (SPR)-based binding assay and showed that our synthetic BCL9-based peptides are capable of fully inhibiting the binding of β-catenin to wild-type BCL9 protein. Our studies provide not only further insight into the interaction between BCL9 and β-catenin but also quantitative and reliable biochemical binding assays for the discovery of potent and specific small-molecule inhibitors of this interaction.