Proteomic Profiling of the Influence of Iron Availability on Cryptococcus gattii
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- 作者：Juliana Crestani ; Paulo Costa Carvalho ; Xuemei Han ; Adriana Seixas ; Leonardo Broetto ; Juliana de Saldanha da Gama Fischer ; Charley Christian Staats ; Augusto Schrank ; John R. Yates ; III ; Marilene Henning Vainstein
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：January 1, 2012
- 年：2012
- 卷：11
- 期：1
- 页码：189-205
- 全文大小：1263K
- 年卷期：v.11,no.1(January 1, 2012)
- ISSN：1535-3907
文摘
Iron is essential and ubiquitous in living organisms. The competition for this micronutrient between the host and its pathogens has been related to disease establishment. Cryptococcus gattii is an encapsulated yeast that causes cryptococcosis mainly in immunocompetent individuals. In this study, we analyzed the proteomic profile of the C. gattii R265 Vancouver Island isolate under iron-depleted and -repleted conditions by multidimensional protein identification technology (MudPIT) and by 2D-GE. Proteins and key mechanisms affected by alteration of iron levels such as capsule production, cAMP-signaling pathway, response to stress, and metabolic pathways related to mitochondrial function were identified. Our results also show both proteomic methodologies employed to be complementary.