Encapsulation of Paclitaxel in Macromolecular Nanoshells

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• 作者：Alisar S. Zahr ; Michael V. Pishko
• 刊名：Biomacromolecules
• 出版年：2007
• 出版时间：June 2007
• 年：2007
• 卷：8
• 期：6
• 页码：2004 - 2010
• 全文大小：603K
• 年卷期：v.8,no.6(June 2007)
• ISSN：1526-4602

文摘

An electrostatic layer-by-layer self-assembly technique was used to encapsulate solid core paclitaxel nanoparticleswithin a polymeric nanometer-scale shell. This approach provides a new strategy for the development of polymericvehicles that control drug release and target diseased tissues and cells specific to the ailment, such as breastcancer. Core paclitaxel nanoparticles, 153 ± 28 nm in diameter, were prepared using a modified nanoprecipitationtechnique. A nanoshell composed of multilayered polyelectrolytes, poly(allylamine hydrochloride) and poly(styrene-4-sulfonate) was assembled stepwise onto core charged drug nanoparticles. In vitro studies were performedto determine the anticancer activity of paclitaxel core-shell nanoparticles. Paclitaxel core-shell nanoparticlesinduced cell cycle arrest in the G2/M phase after 24 and 48 h of incubation with a human breast carcinoma cellline, MCF-7. Changes in MCF-7 cell morphology, fragmentation of the nucleus, and loss of cell-cell contactsindicated that the cells responded to paclitaxel core nanoparticles upon treatment for 24 and 48 h. Cells arrestedin G2/M phase illustrated abnormal microtubule and actin cytoskeleton morphology. The core-shell drugnanoparticles fabricated using this procedure provide a new approach in the delivery of paclitaxel devoid ofCremophor EL, a solvent that causes adverse side effects in patients undergoing chemotherapy for treatment ofmetastasized mammary cancers.