Chaxapeptin, a Lasso Peptide from Extremotolerant Streptomyces leeuwenhoekii Strain C58 from the Hyperarid Atacama Desert
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- 作者：Somayah S. Elsayed ; Franziska Trusch ; Hai Deng ; Andrea Raab ; Ivan Prokes ; Kanungnid Busarakam ; Juan A. Asenjo ; Barbara A. Andrews ; Pieter van West ; Alan T. Bull ; Michael Goodfellow ; Yu Yi ; Rainer Ebel ; Marcel Jaspars ; Mostafa E. Rateb
- 刊名：Journal of Organic Chemistry
- 出版年：2015
- 出版时间：October 16, 2015
- 年：2015
- 卷：80
- 期：20
- 页码：10252-10260
- 全文大小：485K
- ISSN：1520-6904
文摘
Lasso peptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that possess a unique 鈥渓ariat knot鈥?structural motif. Genome mining-targeted discovery of new natural products from microbes obtained from extreme environments has led to the identification of a gene cluster directing the biosynthesis of a new lasso peptide, designated as chaxapeptin 1, in the genome of Streptomyces leeuwenhoekii strain C58 isolated from the Atacama Desert. Subsequently, 1 was isolated and characterized using high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance methods. The lasso nature of 1 was confirmed by calculating its nuclear Overhauser effect restraint-based solution structure. Chaxapeptin 1 displayed a significant inhibitory activity in a cell invasion assay with human lung cancer cell line A549.