Antiadhesive Polymer Brush Coating Functionalized with Antimicrobial and RGD Peptides to Reduce Biofilm Formation and Enhance Tissue Integration
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- 作者：Agnieszka K. Muszanska ; Edward T. J. Rochford ; Agnieszka Gruszka ; Andreas A. Bastian ; Henk J. Busscher ; Willem Norde ; Henny C. van der Mei ; Andreas Herrmann
- 刊名：Biomacromolecules
- 出版年：2014
- 出版时间：June 9, 2014
- 年：2014
- 卷：15
- 期：6
- 页码：2019-2026
- 全文大小：431K
- 年卷期：v.15,no.6(June 9, 2014)
- ISSN：1526-4602
文摘
This paper describes the synthesis and characterization of polymer鈥損eptide conjugates to be used as infection-resistant coating for biomaterial implants and devices. Antiadhesive polymer brushes composed of block copolymer Pluronic F-127 (PF127) were functionalized with antimicrobial peptides (AMP), able to kill bacteria on contact, and arginine鈥揼lycine鈥揳spartate (RGD) peptides to promote the adhesion and spreading of host tissue cells. The antiadhesive and antibacterial properties of the coating were investigated with three bacterial strains: Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. The ability of the coating to support mammalian cell growth was determined using human fibroblast cells. Coatings composed of the appropriate ratio of the functional components: PF127, PF127 modified with AMP, and PF127 modified with RGD showed good antiadhesive and bactericidal properties without hampering tissue compatibility.