Ubiquitous Structural Signaling in Bacterial Phytochromes
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- 作者：Alexander Bj枚rling ; Oskar Berntsson ; Heikki Takala ; Kevin D. Gallagher ; Hardik Patel ; Emil Gustavsson ; Rachael St. Peter ; Phu Duong ; Angela Nugent ; Fan Zhang ; Peter Berntsen ; Roberto Appio ; Ivan Rajkovic ; Heli Lehtivuori ; Matthijs R. Panman ; Maria Hoernke ; Stephan Niebling ; Rajiv Harimoorthy ; Tilman Lamparter ; Emina A. Stojkovi膰 ; Janne A. Ihalainen ; Sebastian Westenhoff
- 刊名：The Journal of Physical Chemistry Letters
- 出版年：2015
- 出版时间：September 3, 2015
- 年：2015
- 卷：6
- 期：17
- 页码：3379-3383
- 全文大小：299K
- ISSN：1948-7185
文摘
The phytochrome family of light-switchable proteins has long been studied by biochemical, spectroscopic and crystallographic means, while a direct probe for global conformational signal propagation has been lacking. Using solution X-ray scattering, we find that the photosensory cores of several bacterial phytochromes undergo similar large-scale structural changes upon red-light excitation. The data establish that phytochromes with ordinary and inverted photocycles share a structural signaling mechanism and that a particular conserved histidine, previously proposed to be involved in signal propagation, in fact tunes photoresponse.