Rapid Determination of Enantiomeric Excess Using Infrared Thermography

详细信息    查看全文

• 作者：Nicolas Millot ; Phil Borman ; Mike S. Anson ; Ian B. Campbell ; Simon J. F. Macdonald ; and Mahmoud Mahmoudian
• 刊名：Organic Process Research & Development
• 出版年：2002
• 出版时间：July 2002
• 年：2002
• 卷：6
• 期：4
• 页码：463 - 470
• 全文大小：306K
• 年卷期：v.6,no.4(July 2002)
• ISSN：1520-586X

文摘

Infrared thermography (IRT) is presented as a novel techniqueto screen a potentially large number of asymmetric catalystsor substrates in a high-throughput fashion. IRT was used as asimple, rapid, and practical approach for initial screening ofthe substrate specificity of Candida antarctica lipase. This wascarried out using a 96-well microtitre plate format. Potentialadvantages and limitations of IRT for the enzymatic stereoselective acylation of primary and secondary alcohols of interestare discussed.