Azo-Sulforhodamine Dyes: A Novel Class of Broad Spectrum Dark Quenchers

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• 作者：Arnaud Chevalier ; Pierre-Yves Renard ; Anthony Romieu
• 刊名：Organic Letters
• 出版年：2014
• 出版时间：August 1, 2014
• 年：2014
• 卷：16
• 期：15
• 页码：3946-3949
• 全文大小：285K
• ISSN：1523-7052

文摘

A rapid access to a novel class of water-soluble dark quencher dyes was achieved using an azo-coupling reaction between a fluorescent primary arylamine derived from a sulforhodamine 101 scaffold and a tertiary aniline equipped with different bioconjugatable groups. The thus obtained nonfluorescent azo-sulforhodamine hybrids display a broad quenching range spanning the visible to NIR regions. This was demonstrated through the preparation and enzymatic activation of FRET-based fluorogenic substrates of urokinase.