Copolymerization of 2-Carboxyisopropylacrylamide with N-Isopropylacrylamide Accelerates Cell Detachment from Grafted Surfaces by Reducing Temperature
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- 作者：Mitsuhiro Ebara ; Masayuki Yamato ; Motohiro Hirose ; Takao Aoyagi ; Akihiko Kikuchi ; Kiyotaka Sakai ; and Teruo Okano
- 刊名：Biomacromolecules
- 出版年：2003
- 出版时间：March 2003
- 年：2003
- 卷：4
- 期：2
- 页码：344 - 349
- 全文大小：114K
- 年卷期：v.4,no.2(March 2003)
- ISSN：1526-4602
文摘
Acrylic acid (AAc) has been utilized to introduce reactive carboxyl groups to a temperature-responsivepolymer, poly(N-isopropylacrylamide) (PIPAAm). However, AAc introduction shifts the copolymer phasetransition temperatures higher and dampens the steep homopolymer phase transition with increasing AAccontent. We previously synthesized 2-carboxyisopropylacrylamide (CIPAAm) having both a similar sidechain structure to IPAAm and a functional carboxylate group in order to overcome these shortcomings. Inthe present study, these copolymers, grafted onto cell culture plastic, were assessed for cell adhesion controlusing their phase transition. AAc introduction to PIPAAm-grafted surfaces resulted in excessive surfacehydration and hindered cell spreading in culture at 37 
C. In contrast, CIPAAm-containing copolymer-grafted surfaces exhibited relatively weak hydrophobicity similar to both homopolymer PIPAAm-graftedsurfaces as well as commercial ungrafted tissue culture polystyrene dish surfaces. Cells adhered and spreadwell on these surfaces at 37 C in culture. As observed previously on PIPAAm-grafted surfaces, cells werespontaneously detached from the copolymer-grafted surfaces by reducing culture temperature. Cell detachmentwas accelerated on the CIPAAm copolymer-grafted surfaces compared to pure IPAAm surfaces, suggestingthat hydrophilic carboxyl group microenvironment in the monomer and polymer is important to accelerategrafted surface hydration below the lower critical solution temperature, detaching cells.
C. In contrast, CIPAAm-containing copolymer-grafted surfaces exhibited relatively weak hydrophobicity similar to both homopolymer PIPAAm-graftedsurfaces as well as commercial ungrafted tissue culture polystyrene dish surfaces. Cells adhered and spreadwell on these surfaces at 37 C in culture. As observed previously on PIPAAm-grafted surfaces, cells werespontaneously detached from the copolymer-grafted surfaces by reducing culture temperature. Cell detachmentwas accelerated on the CIPAAm copolymer-grafted surfaces compared to pure IPAAm surfaces, suggestingthat hydrophilic carboxyl group microenvironment in the monomer and polymer is important to accelerategrafted surface hydration below the lower critical solution temperature, detaching cells.