Identification of Bacteria by Conjugated Oligoelectrolyte/Single-Stranded DNA Electrostatic Complexes
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- 作者：Aidee Duarte ; Arkadiusz Chworos ; Suvi F. Flagan ; Grady Hanrahan ; Guillermo C. Bazan
- 刊名：Journal of the American Chemical Society
- 出版年：2010
- 出版时间：September 15, 2010
- 年：2010
- 卷：132
- 期：36
- 页码：12562-12564
- 全文大小：157K
- 年卷期：v.132,no.36(September 15, 2010)
- ISSN：1520-5126
文摘
Electrostatic complexes containing a cationic conjugated oligoelectrolyte (COE) and fluorescein (FAM)-labeled single-stranded DNA (ssDNA) serve as the basis for identifying various bacteria. The approach involves the preparation of five COE/ssDNAx-FAM electrostatic complexes, which differ in the ssDNA composition and which provide different photoluminescence (PL) spectra as a result of different degrees of energy transfer efficiency from the COE to FAM. Changes in the PL spectra upon addition of the bacteria can be quantified, and the differential response from the five ssDNAs gives rise to a multicomponent array response that allows identification of the microorganism under investigation.