Thiol-Mediated Anchoring of Ligands to Self-Assembled Monolayers for Studies of Biospecific Interactions

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• 作者：Kunal V. Gujraty ; Randolph Ashton ; Sridhar R. Bethi ; Sandesh Kate ; Christopher J. Faulkner ; G. Kane Jennings ; Ravi S. Kane
• 刊名：Langmuir
• 出版年：2006
• 出版时间：November 21, 2006
• 年：2006
• 卷：22
• 期：24
• 页码：10157 - 10162
• 全文大小：110K
• 年卷期：v.22,no.24(November 21, 2006)
• ISSN：1520-5827

文摘

We report a method to immobilize thiol-containing ligands onto self-assembled monolayers (SAMs) of alkanethiolatespresenting chloracetylated hexa(ethylene glycol) groups. The chloroacetyl groups react with thiols under mild basicconditions, enabling the stable immobilization of biologically active ligands in a well-defined orientation. These SAMson gold are well suited for studies of biospecific interactions of immobilized ligands with proteins and cells. As ademonstration, we functionalized these SAMs with thiol-containing derivatives of biotin and benzene sulfonamideand observed the specific binding of neutravidin and carbonic anhydrase, respectively. We also used this method togenerate mixed SAMs presenting the Arg-Gly-Asp (RGD) peptide sequence and demonstrated the integrin-mediatedadhesion of fibroblast cells to these SAMs. This approach would allow the immobilization of proteins and othersensitive biomolecules and ligands for a wide variety of applications in biotechnology.