Hydrophilic, Bright CuInS2 Quantum Dots as Cd-Free Fluorescent Labels in Quantitative Immunoassay

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• 作者：Elena S. Speranskaya ; Natalia V. Beloglazova ; Sofie Ab茅 ; Tangi Aubert ; Philippe F. Smet ; Dirk Poelman ; Irina Y. Goryacheva ; Sarah De Saeger ; Zeger Hens
• 刊名：Langmuir
• 出版年：2014
• 出版时间：July 1, 2014
• 年：2014
• 卷：30
• 期：25
• 页码：7567-7575
• 全文大小：364K
• ISSN：1520-5827

文摘

We report on the synthesis of core鈥搒hell CuInS₂/ZnS quantum dots (QDs) in organic solution, their encapsulation with a PEG-containing amphiphilic polymer, and the application of the resulting water-soluble QDs as fluorescent label in quantitative immunoassay. By optimizing the methods for core synthesis and shell growth, CuInS₂/ZnS QDs were obtained with a quantum yield of 50% on average after hydrophilization. After conjugation with an aflatoxin B1-protein derivative, the obtained QDs were used as fluorescent labels in microplate immunoassay for the quantitative determination of the mycotoxin aflatoxin B1. QDs-based immunoassay showed higher sensitivity compared to enzyme-based immunoassay.