New Approaches for Bottom-Up Assembly of Tobacco Mosaic Virus-Derived Nucleoprotein Tubes on Defined Patterns on Silica- and Polymer-Based Substrates
详细信息 查看全文
- 作者：Carlos Azucena ; Fabian J. Eber ; Vanessa Trouillet ; Michael Hirtz ; Stefan Heissler ; Matthias Franzreb ; Harald Fuchs ; Christina Wege ; Hartmut Gliemann
- 刊名：Langmuir
- 出版年：2012
- 出版时间：October 23, 2012
- 年：2012
- 卷：28
- 期：42
- 页码：14867-14877
- 全文大小：637K
- 年卷期：v.28,no.42(October 23, 2012)
- ISSN：1520-5827
文摘
The capability of some natural molecular building blocks to self-organize into defined supramolecular architectures is a versatile tool for nanotechnological applications. Their site-selective integration into a technical context, however, still poses a major challenge. RNA-directed self-assembly of tobacco mosaic virus-derived coat protein on immobilized RNA scaffolds presents a possibility to grow nucleoprotein nanotubes in place. Two new methods for their site-selective, bottom-up assembly are introduced. For this purpose, isothiocyanate alkoxysilane was used to activate oxidic surfaces for the covalent immobilization of DNA oligomers, which served as linkers for assembly-directing RNA. Patterned silanization of surfaces was achieved (1) on oxidic surfaces via dip-pen nanolithography and (2) on polymer surfaces (poly(dimethylsiloxane)) via selective oxidization by UV-light irradiation in air. Atomic force microscopy and X-ray photoelectron spectroscopy were used to characterize the surfaces. It is shown for the first time that the combination of the mentioned structuring methods and the isothiocyanate-based chemistry is appropriate (1) for the site-selective immobilization of nucleic acids and, thus, (2) for the formation of viral nanoparticles by bottom-up self-assembly after adding the corresponding coat proteins.