Optimization of Sample Preparation for Peptide Sequencing by MALDI-TOF Photofragment Mass Spectrometry
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- 作者：Justin M. Hettick ; David L. McCurdy ; Damon C. Barbacci ; and David H. Russell
- 刊名：Analytical Chemistry
- 出版年：2001
- 出版时间：November 15, 2001
- 年：2001
- 卷：73
- 期：22
- 页码：5378 - 5386
- 全文大小：109K
- 年卷期：v.73,no.22(November 15, 2001)
- ISSN：1520-6882
文摘
This paper describes the optimization of sample preparation for MALDI 193-nm photofragment ion time-of-flightmass spectrometry to sequence small to medium-sizedpeptides from peptide mixtures. We show that matrixadditives, such as fructose and phenylbutyric acid havea dramatic effect on the abundance of fragment ionsobserved in the post-source decay spectra. A dried-dropletMALDI matrix consisting of 1:1 
-cyano-4-hydroxycinnamic acid/fructose proves to be an excellent matrix forphotodissociation because [M + H]+ ions are formed withlow internal energies, and the photofragment ion spectrum contains high abundances of sequence-informativeions. The addition of fructose appears to improve overallsample homogeneity and durability, as compared toconventional -cyano-4-hydroxycinnamic acid dried-droplet preparations. MALDI-TOF photodissociation isthen used to selectively sequence the peptides bradykinin(RPPGFSPFR), des-Arg9 bradykinin (RPPGFSPF), andsubstance P-amide (RPKPQQFFGLM-NH2) from a mixture of five peptides.
-cyano-4-hydroxycinnamic acid/fructose proves to be an excellent matrix forphotodissociation because [M + H]+ ions are formed withlow internal energies, and the photofragment ion spectrum contains high abundances of sequence-informativeions. The addition of fructose appears to improve overallsample homogeneity and durability, as compared toconventional -cyano-4-hydroxycinnamic acid dried-droplet preparations. MALDI-TOF photodissociation isthen used to selectively sequence the peptides bradykinin(RPPGFSPFR), des-Arg9 bradykinin (RPPGFSPF), andsubstance P-amide (RPKPQQFFGLM-NH2) from a mixture of five peptides.