Identification of Purines and 7-Deazapurines as Potent and Selective Type I Inhibitors of Troponin I-Interacting Kinase (TNNI3K)

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• 作者：Brian G. Lawhorn ; Joanne Philp ; Yongdong Zhao ; Christopher Louer ; Marlys Hammond ; Mui Cheung ; Harvey Fries ; Alan P. Graves ; Lisa Shewchuk ; Liping Wang ; Joshua E. Cottom ; Hongwei Qi ; Huizhen Zhao ; Rachel Totoritis ; Guofeng Zhang ; Benjamin Schwartz ; Hu Li ; Sharon Sweitzer ; Dennis A. Holt ; Gregory J. Gatto ; Jr. ; Lara S. Kallander
• 刊名：Journal of Medicinal Chemistry
• 出版年：2015
• 出版时间：September 24, 2015
• 年：2015
• 卷：58
• 期：18
• 页码：7431-7448
• 全文大小：857K
• ISSN：1520-4804

文摘

A series of cardiac troponin I-interacting kinase (TNNI3K) inhibitors arising from 3-((9H-purin-6-yl)amino)-N-methyl-benzenesulfonamide (1) is disclosed along with fundamental structure鈥揻unction relationships that delineate the role of each element of 1 for TNNI3K recognition. An X-ray structure of 1 bound to TNNI3K confirmed its Type I binding mode and is used to rationalize the structure鈥揳ctivity relationship and employed to design potent, selective, and orally bioavailable TNNI3K inhibitors. Identification of the 7-deazapurine heterocycle as a superior template (vs purine) and its elaboration by introduction of C4-benzenesulfonamide and C7- and C8鈥?-deazapurine substituents produced compounds with substantial improvements in potency (>1000-fold), general kinase selectivity (10-fold improvement), and pharmacokinetic properties (>10-fold increase in poDNAUC). Optimal members of the series have properties suitable for use in in vitro and in vivo experiments aimed at elucidating the role of TNNI3K in cardiac biology and serve as leads for developing novel heart failure medicines.