A Chemical Screening System for Glucocorticoid Stress Hormone Signaling in an Intact Vertebrate

详细信息    查看全文

• 作者：Benjamin D. Weger ; Meltem Weger ; Michael Nusser ; Gerald Brenner-Weiss ; Thomas Dickmeis
• 刊名：ACS Chemical Biology
• 出版年：2012
• 出版时间：July 20, 2012
• 年：2012
• 卷：7
• 期：7
• 页码：1178-1183
• 全文大小：473K
• 年卷期：v.7,no.7(July 20, 2012)
• ISSN：1554-8937

文摘

Glucocorticoids, steroid hormones of the adrenal gland, are an integral part of the stress response and regulate glucose metabolism. Natural and synthetic glucocorticoids are widely used in anti-inflammatory therapy but can have severe side effects. In vivo tests are needed to identify novel glucocorticoids and to screen compounds for unwanted effects on glucocorticoid signaling. We created the Glucocorticoid Responsive In vivoZebrafish Luciferase activitY assay to monitor glucocorticoid signaling in vivo. The GRIZLY assay detects stress-induced glucocorticoid production in single zebrafish larvae, measures disruption of glucocorticoid signaling by an organotin pollutant metabolite, and specifically identifies a compound stimulating endogenous glucocorticoid production in a chemical screen. Our assay has broad applications in stress research, environmental monitoring, and drug discovery.