DHA-Induced Changes of Supported Lipid Membrane Morphology
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- 作者：Dorota Thid ; Jason J. Benkoski ; Sofia Svedhem ; Bengt Kasemo ; Julie Gold
- 刊名：Langmuir
- 出版年：2007
- 出版时间：May 22, 2007
- 年：2007
- 卷：23
- 期：11
- 页码：5878 - 5881
- 全文大小：140K
- 年卷期：v.23,no.11(May 22, 2007)
- ISSN：1520-5827
文摘
Docosahexaenoic acid (DHA) is a polyunsaturated long fatty acid known to have fundamental effects on cellmembrane function. Here, the effect of DHA on phosphocholine-supported lipid bilayers was measured using thequartz crystal microbalance with dissipation monitoring (QCM-D) technique. Above a concentration of 60 
M (i.e.,near the critical micelle concentration), DHA had drastic effects on the viscoelastic properties of the supportedmembranes, suggesting a more complex process and structure than simple insertion of molecules in the bilayer.Fluorescence microscopy revealed the spontaneous formation of elongated out-growths from the bilayers, which wereremarkable for their length (~100 m) and extensive coverage of the surface. These results demonstrate the applicabilityof QCM-D as a method to screen for conditions where membrane remodeling occurs but also that complementarytechniques are required to describe in more detail the changes in viscoelastic properties of the membrane. These resultsare highly relevant for the present rapid development in the field of model lipid membranes aiming toward increasedknowledge about processes occurring at biological surfaces.
M (i.e.,near the critical micelle concentration), DHA had drastic effects on the viscoelastic properties of the supportedmembranes, suggesting a more complex process and structure than simple insertion of molecules in the bilayer.Fluorescence microscopy revealed the spontaneous formation of elongated out-growths from the bilayers, which wereremarkable for their length (~100 m) and extensive coverage of the surface. These results demonstrate the applicabilityof QCM-D as a method to screen for conditions where membrane remodeling occurs but also that complementarytechniques are required to describe in more detail the changes in viscoelastic properties of the membrane. These resultsare highly relevant for the present rapid development in the field of model lipid membranes aiming toward increasedknowledge about processes occurring at biological surfaces.