Capturing a Sulfenic Acid with Arylboronic Acids and Benzoxaborole
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- 作者：C. Tony Liu ; Stephen J. Benkovic
- 刊名：Journal of the American Chemical Society
- 出版年：2013
- 出版时间：October 2, 2013
- 年：2013
- 卷：135
- 期：39
- 页码：14544-14547
- 全文大小：261K
- 年卷期：v.135,no.39(October 2, 2013)
- ISSN：1520-5126
文摘
Post-translational redox generation of cysteine-sulfenic acids (Cys-SOH) functions as an important reversible regulatory mechanism for many biological functions, such as signal transduction, balancing cellular redox states, catalysis, and gene transcription. Herein we show that arylboronic acids and cyclic benzoxaboroles can form adducts with sulfenic acids in aqueous medium and that these boron-based compounds can potentially be used to trap biologically significant sulfenic acids. As proof of principle we demonstrate that a benzoxaborole can inhibit the enzyme activity of an iron-containing nitrile hydratase, which requires a catalytic 伪Cys114-SOH in the active site. The nature of the adduct and the effect of the boronic acid鈥檚 pKaB on the stability constant of the adduct are discussed within.