Expanding the Structural Diversity of Polyketides by Exploring the Cofactor Tolerance of an Inline Methyltransferase Domain

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• 作者：Jaclyn M. Winter ; Grace Chiou ; Ian R. Bothwell ; Wei Xu ; Neil K. Garg ; Minkui Luo ; Yi Tang
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 19, 2013
• 年：2013
• 卷：15
• 期：14
• 页码：3774-3777
• 全文大小：241K
• 年卷期：v.15,no.14(July 19, 2013)
• ISSN：1523-7052

文摘

A strategy for introducing structural diversity into polyketides by exploiting the promiscuity of an in-line methyltransferase domain in a multidomain polyketide synthase is reported. In vitro investigations using the highly-reducing fungal polyketide synthase CazF revealed that its methyltransferase domain accepts the nonnatural cofactor propargylic Se-adenosyl-l-methionine and can transfer the propargyl moiety onto its growing polyketide chain. This propargylated polyketide product can then be further chain-extended and cyclized to form propargyl-伪 pyrone or be processed fully into the alkyne-containing 4鈥?propargyl-chaetoviridin A.