Real-Time Bioluminescence Imaging of Nitroreductase in Mouse Model

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• 作者：Ping Feng ; Huateng Zhang ; Quankun Deng ; Wei Liu ; Linghui Yang ; Guobo Li ; Guo Chen ; Lupei Du ; Bowen Ke ; Minyong Li
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：June 7, 2016
• 年：2016
• 卷：88
• 期：11
• 页码：5610-5614
• 全文大小：322K
• 年卷期：0
• ISSN：1520-6882

文摘

Nitroreductase (NTR) is an endogenous reductase overexpressed in hypoxic tumors; however, its precise detection in living cells and animals remains a considerable challenge. Herein, we developed three reaction-based probes and a related bioluminescence assay for the real-time NTR detection. The high sensitivity and selectivity of probe 3, combined with its remarkable potential of bioluminescence imaging, affords a valuable approach for in vivo imaging of NTR in a tumor model mouse.