Attenuation of Mouse Melanoma by A/C Magnetic Field after Delivery of Bi-Magnetic Nanoparticles by Neural Progenitor Cells

详细信息    查看全文

• 作者：Raja Shekar Rachakatla ; Sivasai Balivada ; Gwi-Moon Seo ; Carl B. Myers ; Hongwang Wang ; Thilani N. Samarakoon ; Raj Dani ; Marla Pyle ; Franklin O. Kroh ; Brandon Walker ; Xiaoxuan Leaym ; Olga B. Koper ; Viktor Chikan ; Stefan H. Bossmann ; Masaaki Tamura ; Deryl L. Troyer
• 刊名：ACS Nano
• 出版年：2010
• 出版时间：December 28, 2010
• 年：2010
• 卷：4
• 期：12
• 页码：7093-7104
• 全文大小：649K
• 年卷期：0
• ISSN：1936-086X

文摘

Localized magnetic hyperthermia as a treatment modality for cancer has generated renewed interest, particularly if it can be targeted to the tumor site. We examined whether tumor-tropic neural progenitor cells (NPCs) could be utilized as cell delivery vehicles for achieving preferential accumulation of core/shell iron/iron oxide magnetic nanoparticles (MNPs) within a mouse model of melanoma. We developed aminosiloxane−porphyrin functionalized MNPs, evaluated cell viability and loading efficiency, and transplanted neural progenitor cells loaded with this cargo into mice with melanoma. NPCs were efficiently loaded with core/shell Fe/Fe₃O₄ MNPs with minimal cytotoxicity; the MNPs accumulated as aggregates in the cytosol. The NPCs loaded with MNPs could travel to subcutaneous melanomas, and after A/C (alternating current) magnetic field (AMF) exposure, the targeted delivery of MNPs by the cells resulted in a measurable regression of the tumors. The tumor attenuation was significant (p < 0.05) a short time (24 h) after the last of three AMF exposures.