Synthesis of Sugar Arrays in Microtiter Plate
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- 作者：Fabio Fazio ; Marian C. Bryan ; Ola Blixt ; James C. Paulson ; and Chi-Huey Wong
- 刊名：Journal of the American Chemical Society
- 出版年：2002
- 出版时间：December 4, 2002
- 年：2002
- 卷：124
- 期：48
- 页码：14397 - 14402
- 全文大小：183K
- 年卷期：v.124,no.48(December 4, 2002)
- ISSN：1520-5126
文摘
1,3-Dipolar cycloadditions between azides and alkynes were exploited to attach oligosaccharidesto a C14 hydrocarbon chain that noncovalently binds to the microtiter well surface. Synthesis of sugar arrayswas performed on a micromolar scale in situ in the microtiter plate. As a model study, the 
ddle">-galactosyllipid5 was displayed on a 4-
mol scale. Formation of product was confirmed via ESI-MS, and the yield wasdetermined via chemical and biological assays. Several complex carbohydrates (6-16) were also displayedin microtiter plates and successfully screened with various lectins. Moreover, sialyl Lewis x (17) wassynthesized via the enzymatic fucosylation of a precursor displayed in the plate. Studies on inhibition ofthis biotransformation have been carried out, and the IC50 value found for the known inhibitor 20 wasconsistent with previous studies in solution.
ddle">-galactosyllipid5 was displayed on a 4-mol scale. Formation of product was confirmed via ESI-MS, and the yield wasdetermined via chemical and biological assays. Several complex carbohydrates (6-16) were also displayedin microtiter plates and successfully screened with various lectins. Moreover, sialyl Lewis x (17) wassynthesized via the enzymatic fucosylation of a precursor displayed in the plate. Studies on inhibition ofthis biotransformation have been carried out, and the IC50 value found for the known inhibitor 20 wasconsistent with previous studies in solution.