Bioactive Constituents of Indigofera spicata

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• 作者：Lynette Bueno P茅rez ; Jie Li ; Daniel D. Lantvit ; Li Pan ; Tran Ngoc Ninh ; Hee-Byung Chai ; Djaja Djendoel Soejarto ; Steven M. Swanson ; David M. Lucas ; A. Douglas Kinghorn
• 刊名：Journal of Natural Products
• 出版年：2013
• 出版时间：August 23, 2013
• 年：2013
• 卷：76
• 期：8
• 页码：1498-1504
• 全文大小：306K
• 年卷期：v.76,no.8(August 23, 2013)
• ISSN：1520-6025

文摘

Four new flavanones, designated as (+)-5鈥?deacetylpurpurin (1), (+)-5-methoxypurpurin (2), (2S)-2,3-dihydrotephroglabrin (3), and (2S)-2,3-dihydrotephroapollin C (4), together with two known flavanones (5 and 6), three known rotenoids (7鈥?b>9), and one known chalcone (10) were isolated from a chloroform-soluble partition of a methanol extract from the combined flowers, fruits, leaves, and twigs of Indigofera spicata, collected in Vietnam. The compounds were obtained by bioactivity-guided isolation using the HT-29 human colon cancer, 697 human acute lymphoblastic leukemia, and Raji human Burkitt鈥檚 lymphoma cell lines. The structures of 1鈥?b>4 were established by extensive 1D- and 2D-NMR experiments, and the absolute configurations were determined by the measurement of specific rotations and CD spectra. The cytotoxic activities of the isolated compounds were tested against the HT-29, 697, Raji, and CCD-112CoN human normal colon cells. Also, the quinone reductase induction activities of the isolates were determined using the Hepa 1c1c7 murine hepatoma cell line. In addition, cis-(6a尾,12a尾)-hydroxyrotenone (7) was evaluated in an in vivo hollow fiber bioassay using HT-29, MCF-7 human breast cancer, and MDA-MB-435 human melanoma cells.