Selective Uncoupling of Individual Mitochondria within a Cell Using a Mitochondria-Targeted Photoactivated Protonophore

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• 作者：Susan Chalmers ; Stuart T. Caldwell ; Caroline Quin ; Tracy A. Prime ; Andrew M. James ; Andrew G. Cairns ; Michael P. Murphy ; John G. McCarron ; Richard C. Hartley
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：January 18, 2012
• 年：2012
• 卷：134
• 期：2
• 页码：758-761
• 全文大小：274K
• 年卷期：v.134,no.2(January 18, 2012)
• ISSN：1520-5126

文摘

Depolarization of an individual mitochondrion or small clusters of mitochondria within cells has been achieved using a photoactivatable probe. The probe is targeted to the matrix of the mitochondrion by an alkyltriphenylphosphonium lipophilic cation and releases the protonophore 2,4-dinitrophenol locally in predetermined regions in response to directed irradiation with UV light via a local photolysis system. This also provides a proof of principle for the general temporally and spatially controlled release of bioactive molecules, pharmacophores, or toxins to mitochondria with tissue, cell, or mitochondrion specificity.