Assessment of Specific Binding Proteins Suitable for the Detection of Paralytic Shellfish Poisons Using Optical Biosensor Technology

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• 作者：Katrina Campbell ; Linda D. Stewart ; Gregory J. Doucette ; Terence L. Fodey ; Simon A. Haughey ; Natalia Vilariñ ; o ; Kentaro Kawatsu ; Christopher T. Elliott
• 刊名：Analytical Chemistry
• 出版年：2007
• 出版时间：August 1, 2007
• 年：2007
• 卷：79
• 期：15
• 页码：5906 - 5914
• 全文大小：165K
• 年卷期：v.79,no.15(August 1, 2007)
• ISSN：1520-6882

文摘

Paralytic shellfish poisoning (PSP) toxin monitoring inshellfish is currently performed using the internationallyaccredited AOAC mouse bioassay. Due to ethical andperformance-related issues associated with this bioassay,the European Commission has recently published directives extending procedures that may be used for officialPSP control. The feasibility of using a surface plasmonresonance optical biosensor to detect PSP toxins inshellfish tissue below regulatory levels was examined.Three different PSP toxin protein binders were investigated: a sodium channel receptor (SCR) preparationderived from rat brains, a monoclonal antibody (GT13-A) raised to gonyautoxin 2/3, and a rabbit polyclonalantibody (R895) raised to saxitoxin (STX). Inhibitionassay formats were used throughout. Immobilization ofSTX to the biosensor chip surface was achieved via amino-coupling. Specific binding and inhibition of binding to thissurface was achieved using all proteins tested. For STXcalibration curves, 0-1000 ng/mL, IC₅₀ values for eachbinder were as follows: SCR 8.11 ng/mL; GT13-A 5.77ng/mL; and R895 1.56 ng/mL. Each binder demonstrated a different cross-reactivity profile against a rangeof STX analogues. R895 delivered a profile that was mostlikely to detect the widest range of PSP toxins at or belowthe internationally adopted regulatory limits.